State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Viruses. 2022 Aug 6;14(8):1737. doi: 10.3390/v14081737.
The nervous necrosis virus (NNV) mainly attacks the central nervous system of fish to cause viral nervous necrosis, which is an acute and serious prevalent disease in fish. Among different genotypes of NNV, red-spotted grouper nervous necrosis virus (RGNNV) is the most widely reported, with the highest number of susceptible species. To better understand the pathogenicity of RGNNV, we first developed a reverse genetic system for recombinant RGNNV rescue using B7GG and striped snakehead (SSN-1) cells. Furthermore, we constructed attenuated RGNNV strains rRGNNV-B2-M1 and rRGNNV-B2-M2 with the loss of B2 protein expression, which grew slower and induced less Mx1 expression than that of wild-type RGNNV. Moreover, rRGNNV-B2-M1 and rRGNNV-B2-M2 were less virulent than the wild-type RGNNV. Our study provides a potential tool for further research on the viral protein function, virulence pathogenesis, and vaccine development of RGNNV, which is also a template for the rescue of other fish viruses.
神经坏死病毒（NNV）主要攻击鱼类的中枢神经系统，引起病毒性神经坏死，这是鱼类中一种急性且严重的流行疾病。在不同基因型的 NNV 中，红鳍东方鲀神经坏死病毒（RGNNV）是报道最多的，易感物种最多。为了更好地了解 RGNNV 的致病性，我们首先使用 B7GG 和条纹尖吻鲈（SSN-1）细胞开发了用于重组 RGNNV 拯救的反向遗传系统。此外，我们构建了具有 B2 蛋白表达缺失的减毒 RGNNV 株 rRGNNV-B2-M1 和 rRGNNV-B2-M2，它们的生长速度比野生型 RGNNV 慢，诱导的 Mx1 表达也较少。此外，rRGNNV-B2-M1 和 rRGNNV-B2-M2 的毒力比野生型 RGNNV 低。我们的研究为进一步研究 RGNNV 的病毒蛋白功能、毒力发病机制和疫苗开发提供了一个潜在的工具，这也是拯救其他鱼类病毒的模板。
