Volesky B, Zajic J E, Carroll K K
J Nutr. 1975 Mar;105(3):311-6. doi: 10.1093/jn/105.3.311.
Preliminary feeding studies were conducted with a high protein fungus (Graphium sp.) grown on natural gas. The biomass, containing approximately 50% protein, is considered a potential source of single cell protein. In short-term (1-week) experiments, rats gained weight when fed semisynthetic diets containing up to 40% of the biomass, although growth was depressed at the higher levels. Rats lost weight but survived for this length of time when fed biomass alone. Chronic toxicity was tested in a 5-month study with diets in which the casein (20% by weight) of the semisynthetic diet was either partially or entirely replaced by the proteinaceous biomass. Rats fed these diets grew more slowly but appeared normal and healthy, and no pathological changes were observed at autopsy. Some of these rats were fed a standard laboratory diet for an additional 10-month observation period, and at autopsy, one cortical adenoma of the kidney was found in a rat originally fed the lower level of Graphium biomass.
对一种以天然气为培养基生长的高蛋白真菌（炭角菌属）进行了初步喂养研究。这种含有约50%蛋白质的生物质被视为单细胞蛋白质的潜在来源。在短期（1周）实验中，给大鼠喂食含有高达40%该生物质的半合成日粮时，大鼠体重增加，不过在较高含量水平时生长受到抑制。当仅喂食生物质时，大鼠体重减轻，但在这段时间内存活下来。在一项为期5个月的研究中对慢性毒性进行了测试，在半合成日粮中，用这种含蛋白质的生物质部分或全部替代了酪蛋白（占日粮重量的20%）。喂食这些日粮的大鼠生长较慢，但看起来正常且健康，尸检时未观察到病理变化。其中一些大鼠在额外的10个月观察期内喂食标准实验室日粮，尸检时，在一只最初喂食较低水平炭角菌生物质的大鼠体内发现了一个肾皮质腺瘤。
