Sápi Zoltán, Szapanidisz Jorgosz, Tóth Bernadette, Bodrogi István
St. John's Hospital, Department of Pathology, Budapest, Hungary.
Pathol Oncol Res. 1995;1(1):71-74. doi: 10.1007/BF02893587.
Nuclear extract and image cytometry was used to determine the DNA ploidy pattern of 31 pure seminomas. At least 5-year but usually 10-year follow-up was available to compare the clinical outcome to the DNA ploidy pattern. In 24 cases (77.4%) the DNA indexes (DI) showed tetraploid (1,8 < DI < 2,2) pattern confirming the recent cytogenetic and flow cytometric DNA studies of others. However, in 7 cases (22,6%) the tumors were aneuploid. Out of these aneuploid cases 2 had two subpopulations and 2 had less than 1,8 DI. These latter cases (providing the loss of chromosomal DNA) had elevated AFP levels in serum that raises the question of nonseminomatous transformation without any morphological evidence. Usually, the aneuploid cases had worse prognoses but there was no significant difference. Because of the small number of aneuploid cases wider clinicopathologic studies are required to confirm our results.
采用核提取物和图像细胞术来确定31例纯精原细胞瘤的DNA倍体模式。至少有5年但通常为10年的随访资料，以便将临床结果与DNA倍体模式进行比较。在24例（77.4%）中，DNA指数（DI）显示为四倍体（1.8 < DI < 2.2）模式，这证实了其他人最近进行的细胞遗传学和流式细胞术DNA研究。然而，在7例（22.6%）中，肿瘤为非整倍体。在这些非整倍体病例中，2例有两个亚群，2例DI小于1.8。后一种情况（提示染色体DNA缺失）血清甲胎蛋白水平升高，这就提出了在无任何形态学证据的情况下非精原细胞瘤转化的问题。通常，非整倍体病例预后较差，但无显著差异。由于非整倍体病例数量较少，需要更广泛的临床病理研究来证实我们的结果。
