布琼布拉（布隆迪）1型艾滋病毒毒株的基因分析：C亚型变体占主导地位。

Genetic analysis of hiv type 1 strains in bujumbura (burundi): predominance of subtype c variant.

作者信息

Koch N, Ndihokubwayo J B, Yahi N, Tourres C, Fantini J, Tamalet C

机构信息

Laboratoire de Virologie, CHRU La Timone, 13005 Marseille, France.

出版信息

AIDS Res Hum Retroviruses. 2001 Feb 10;17(3):269-73. doi: 10.1089/088922201750063205.

DOI:10.1089/088922201750063205

PMID:11177411

Abstract

In order to characterize the HIV-1 strains circulating in Burundi, 18 blood samples from nontreated patients were collected in Bujumbura and viral DNA and RNA were sequenced in the env and pol genes, respectively. The phylogenetic analysis of the V3 coding region of HIV-1 gp120 revealed that 83% (15/18) of the isolates belonged to the C subtype. The RT and protease coding regions of the pol gene also clustered with subtype C. A potential A/C recombinant between the protease (subtype A) and the RT and V3 coding regions (both subtype C) was identified. Drug resistance mutations were not detected in the RT gene. However, mutation M36I, associated with resistance to ritonavir and nelfinavir, was found in 17 of 18 Burundi isolates. In conclusion, this first characterization of HIV-1 strains circulating in Burundi confirms the dramatic emergence of subtype C in East Africa.

摘要

为了鉴定布隆迪流行的HIV-1毒株特征，在布琼布拉采集了18份未接受治疗患者的血样，分别对env和pol基因中的病毒DNA和RNA进行测序。HIV-1 gp120 V3编码区的系统发育分析显示，83%（15/18）的分离株属于C亚型。pol基因的RT和蛋白酶编码区也聚类为C亚型。鉴定出一种蛋白酶（A亚型）与RT及V3编码区（均为C亚型）之间潜在的A/C重组体。在RT基因中未检测到耐药性突变。然而，在18株布隆迪分离株中的17株发现了与对利托那韦和奈非那韦耐药相关的M36I突变。总之，对布隆迪流行的HIV-1毒株的首次鉴定证实了C亚型在东非的急剧出现。