Thomson M M, Delgado E, Manjón N, Ocampo A, Villahermosa M L, Mariño A, Herrero I, Cuevas M T, Vázquez-de Parga E, Pérez-Alvarez L, Medrano L, Taboada J A, Nájera R
Area de Patogenia Viral, Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.
AIDS. 2001 Mar 9;15(4):509-16. doi: 10.1097/00002030-200103090-00010.
The HIV-1 epidemics in Western Europe are dominated by B subtype viruses. Non-B subtype is largely restricted to individuals infected outside of Europe and to their direct contacts and is generally acquired by the heterosexual route.
Protease and a segment of reverse transcriptase were amplified and sequenced from plasma RNA in 451 individuals from seven cities of Galicia, north-western Spain. Subtype sequence homologies were determined using the BLAST algorithm. Non-B sequences were examined by phylogenetic analysis and intersubtype recombination by bootscanning. The env V3 region was analysed in all non-B and in 38 B subtype viruses.
Ten different non-B genetic forms were identified in 20 (4.4%) individuals. Subtypes were concordant between pol and V3 in five viruses; 14 (70%) infections were with intersubtype recombinant viruses, and one individual had a dual B+G infection. Seven recombinant viruses were phylogenetically related to five reported recombinant forms. Three non-recombinant G and six recombinant BG viruses formed a monophyletic cluster for pol. All but three individuals with non-B infections were native Spanish. Only 6 of 16 individuals referred to sexual contacts with sub-Saharan Africans. Twelve (60%) non-B subtype infections, including all with G and BG viruses, were in injecting drug users (IDU).
Non-B subtype viruses were identified in 4.4%, with a high diversity of genetic forms, including 70% infections with intersubtype recombinant viruses. The majority of individuals with non-B infections were IDU, most of them without known contacts with non-European sources, and among whom BG recombinant viruses are circulating.
西欧的HIV-1流行主要由B亚型病毒主导。非B亚型主要局限于在欧洲境外感染的个体及其直接接触者，通常通过异性传播途径感染。
从西班牙西北部加利西亚七个城市的451名个体的血浆RNA中扩增并测序蛋白酶和一段逆转录酶。使用BLAST算法确定亚型序列同源性。通过系统发育分析检查非B序列，并通过自展扫描检查亚型间重组。对所有非B亚型和38种B亚型病毒的env V3区域进行分析。
在20名（4.4%）个体中鉴定出10种不同的非B基因形式。5种病毒的pol和V3亚型一致；14例（70%）感染为亚型间重组病毒，1例个体为B+G双重感染。7种重组病毒在系统发育上与5种已报道的重组形式相关。3种非重组G病毒和6种重组BG病毒形成了pol的单系簇。除3名个体外，所有非B感染个体均为西班牙本地人。16名提及与撒哈拉以南非洲人有性接触的个体中只有6名。12例（60%）非B亚型感染，包括所有G和BG病毒感染，发生在注射吸毒者（IDU）中。
4.4%的个体中鉴定出非B亚型病毒，基因形式高度多样，包括70%的亚型间重组病毒感染。大多数非B感染个体为IDU，其中大多数与非欧洲来源无已知接触，且BG重组病毒在他们中间传播。
