Coniglio J G, Grogan W M, Harris D G, Fitzhugh M L
Lipids. 1975 Feb;10(2):109-12. doi: 10.1007/BF02532165.
Testes of quaking mice (sterile mutants) and of controls were analyzed for major lipid classes and fatty acid composition. Of the main lipid classes, only cholesterol esters differed significantly in concentration between the two groups (1.01 for quakers vs 0.69 mg/g wet wt of tissue for controls). The concentration of triglycerides was 4.5-5.0 that of total phosphatides 18-19 and that of free cholesterol 1.9-2.0 mg/g for mutants and controls. The concentrations of phosphatidyl ethanolmanine and of sphingomyelin were both lower in quaking than in normal mice, but only the change in the former was statistically significant. Phosphatidyl choline was the major phosphatide (43-45% of total phosphatides) followed by phosphatidyl ethanolamine (24-26%) and sphingomyelin, phosphatidyl serine, and phosphatidyl inositol (all ca. 7% of total phosphatides). Minor differences between the mutants and controls were observed in concentrations of fatty acids of major lipid classes. The mutants, sterile because of faulty spermatid differentiation, had normal quantities of 22:6 w3 and 22:5 w6. These data are consistent with the hypothesis that the 22-carbon polyenes are associated with the formation of spermatids, rather than with their final differentiation into spermatozoa.
对颤抖小鼠（不育突变体）和对照小鼠的睾丸进行了主要脂质类别和脂肪酸组成分析。在主要脂质类别中，两组之间仅胆固醇酯浓度存在显著差异（颤抖小鼠为1.01，对照小鼠为0.69毫克/克组织湿重）。突变体和对照小鼠的甘油三酯浓度是总磷脂的4.5 - 5.0倍，游离胆固醇浓度为1.9 - 2.0毫克/克。颤抖小鼠中磷脂酰乙醇胺和鞘磷脂的浓度均低于正常小鼠，但只有前者的变化具有统计学意义。磷脂酰胆碱是主要的磷脂（占总磷脂的43 - 45%），其次是磷脂酰乙醇胺（24 - 26%）以及鞘磷脂、磷脂酰丝氨酸和磷脂酰肌醇（均约占总磷脂的7%）。在主要脂质类别的脂肪酸浓度方面，突变体和对照小鼠之间观察到细微差异。由于精子细胞分化缺陷而不育的突变体，其22:6 w3和22:5 w6的含量正常。这些数据与以下假设一致，即22碳多烯与精子细胞的形成有关，而不是与它们最终分化为精子有关。
