锁核酸（LNA）：优化对DNA和RNA的识别

Locked nucleic acid (LNA): fine-tuning the recognition of DNA and RNA.

作者信息

Braasch D A, Corey D R

机构信息

Departments of Pharmacology and Biochemistry, University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390-9041, USA.

出版信息

Chem Biol. 2001 Jan;8(1):1-7. doi: 10.1016/s1074-5521(00)00058-2.

DOI:10.1016/s1074-5521(00)00058-2

PMID:11182314

Abstract

Locked nucleic acid is an RNA derivative in which the ribose ring is constrained by a methylene linkage between the 2'-oxygen and the 4'-carbon. This conformation restriction increases binding affinity for complementarity sequences and provides an exciting new chemical approach for the control of gene expression and optimization of microarrays.

摘要

锁核酸是一种核糖核酸衍生物，其中核糖环通过2'-氧原子与4'-碳原子之间的亚甲基连接而受到限制。这种构象限制增加了对互补序列的结合亲和力，并为基因表达的控制和微阵列的优化提供了一种令人兴奋的新化学方法。