[对一株重组炭疽芽孢杆菌毒力的研究，该重组菌株是通过从一株蜡样芽孢杆菌进行染色体基因转导转移而获得的]
[study of the virulence of a recombinant strain of Bacillus anthracis, obtained as a result of transductive transfer of chromosomal genes from a strain of Bacillus cereus].
作者信息
Mikshis N I, Bolotnikova M F, Novikova L V
机构信息
Mikrob Russian Plague Control Institute, Saratov, Russia.
出版信息
Mol Gen Mikrobiol Virusol. 2000(4):12-5.
Auxotrophic markers of B. anthracis strains differing them from other Bacillus representatives have been determined. Chromosome genes from prototrophic B. cereus strain were transduced into auxotrophic B. anthracis strain. The properties of transductants were studied in order to establish common transfer of chromosomal determinants responsible for realization of various signs. Transduction mating between species resulted in construction of prototroph B. anthracis strains (pX01- pX02+), whose derivatives are characterized by decreased virulence for laboratory animals.
已确定了炭疽芽孢杆菌菌株与其他芽孢杆菌代表菌株不同的营养缺陷型标记。将原养型蜡样芽孢杆菌菌株的染色体基因转导到营养缺陷型炭疽芽孢杆菌菌株中。为了确定负责各种特征实现的染色体决定因子的共同转移，研究了转导子的特性。种间转导交配产生了原养型炭疽芽孢杆菌菌株（pX01 - pX02 +），其衍生物对实验动物的毒力降低。
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