Zhao M, Chen J, Yang P
Department of Ophthalmology, First Teaching Hospital, Guangzhou 510060, China.
Yan Ke Xue Bao. 1997 Jun;13(2):70-4.
To investigate immunological mechanism of corneal melting perforation and inflammation after thermal burn.
The models of severe corneal burns were induced with thermal metal in rats. Immunohistochemistry was carried out on wholemounts of the cornea, iris, choroid-sclera comples, retina at different time point after corneal burns, to derect T-lymphocytes and their subpopulation, macrophages, dendritic cells, MHC class II-positive cells.
In the early stage of the burns, there were small number of increasing T-lymphocytes, MHC class II-positive cells in the cornea and iris. At the period of corneal melting and perforation, a massive influx of lymphocytes (CD3, CD4, CD8), macrophages and dentritic cells were seen in the cornea adjacent to limbers and iris. CD4 positive cells were much more than CD8 in number obviously. MHC class II-positive cell were found not only at the limbus but also in the melting area and corneal centre. Morphological changes of these cells were also noted. At restoration stage positive cells decreased gradually.
Immunological mechanism is involved in the pathogenesis of the damage induced with corneal thermal burns, especially in the pathogenesis of corneal melting and perforation after burn.
探讨热烧伤后角膜溶解穿孔及炎症的免疫机制。
用热金属诱导大鼠严重角膜烧伤模型。在角膜烧伤后的不同时间点，对角膜、虹膜、脉络膜 - 巩膜复合体、视网膜进行全层免疫组织化学检测，以检测T淋巴细胞及其亚群、巨噬细胞、树突状细胞、MHC II类阳性细胞。
烧伤早期，角膜和虹膜中有少量T淋巴细胞、MHC II类阳性细胞增多。在角膜溶解穿孔期，在角膜缘和虹膜附近的角膜中可见大量淋巴细胞（CD3、CD4、CD8）、巨噬细胞和树突状细胞涌入。CD4阳性细胞数量明显多于CD8。MHC II类阳性细胞不仅在角膜缘发现，还在溶解区和角膜中心发现。还观察到这些细胞的形态变化。在修复期阳性细胞逐渐减少。
免疫机制参与角膜热烧伤所致损伤的发病机制，尤其是烧伤后角膜溶解穿孔的发病机制。
