[全反式维甲酸或三氧化二砷治疗急性早幼粒细胞白血病期间的组织因子表达]
[Tissue factor expression during all-trans retinoic acid or arsenic trioxide treatment in acute promyelocytic leukemia].
作者信息
Zhao W, Wang H, Guo W
机构信息
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025.
出版信息
Zhonghua Xue Ye Xue Za Zhi. 1998 Sep;19(9):473-6.
OBJECTIVE
In order to study the effect of all-trans retinoic acid (ATRA) or arsenic trioxide (As2O3) treatment on the expression of tissue factor (TF) in acute promyelocytic leukemia(APL).
METHODS
The plasma level of soluble fibrin monomer complex(SFMC) and D-dimer(D-D), and the TF level of cell lysate were measured by ELISA, the transcription of TF mRNA was assessed by RT-PCR.
RESULTS
The plasma level of SFMC and D-D, the procoagulant activity(PCA) of bone marrow blasts, the TF level of cell lysate and the transcription of TF mRNA all remarkably elevated at diagnosis, while reduced after ATRA or As2O3 therapy.
CONCLUSION
Both ATRA and As2O3 downregulated the expression of TF mRNA, decreased the PCA and TF levels in APL cells, inhibited coagulation activation and secondary hyperfibrinolysis, thus greatly relieved the bleeding symptom in the early stage of treatment.
目的
研究全反式维甲酸（ATRA）或三氧化二砷（As2O3）治疗对急性早幼粒细胞白血病（APL）组织因子（TF）表达的影响。
方法
采用酶联免疫吸附测定法（ELISA）检测血浆可溶性纤维蛋白单体复合物（SFMC）和D - 二聚体（D - D）水平，以及细胞裂解液中的TF水平，通过逆转录 - 聚合酶链反应（RT - PCR）评估TF mRNA的转录情况。
结果
诊断时，血浆SFMC和D - D水平、骨髓原始细胞的促凝活性（PCA）、细胞裂解液中的TF水平以及TF mRNA的转录均显著升高，而在ATRA或As2O3治疗后降低。
结论
ATRA和As2O3均可下调TF mRNA的表达，降低APL细胞中的PCA和TF水平，抑制凝血激活和继发性高纤维蛋白溶解，从而在治疗早期极大地缓解出血症状。
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