99mTc-甲氧基异丁基异腈、99mTc-替曲膦和99mTc-呋膦在人软组织肉瘤细胞系中的摄取比较。

Comparative 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin uptake in human soft tissue sarcoma cell lines.

作者信息

Rodriques M, Chehne F, Kalinowska W, Zielinski C, Sinzinger H

机构信息

Department of Nuclear Medicine, University Hospital, Vienna, Austria.

出版信息

Eur J Nucl Med. 2000 Dec;27(12):1839-43.

PMID:11189948

Abstract

The uptake characteristics of technetium-99m hexakis-2-methoxyisobutylisonitrile (MIBI), 99mTc-tetrofosmin and 99mTc-furifosmin in human soft tissue sarcoma cell lines were investigated and compared. After 10-120 min of incubation at 37 degrees C, 32 degrees C and 22 degrees C with 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin, the kinetics of cellular uptake of these tracers in human soft tissue sarcoma cells SW 684 (fibrosarcoma), SW 872 (liposarcoma), SW 982 (synovial sarcoma) and SW 1353 (chondrosarcoma) was assessed. The uptake of 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin was temperature dependent. The kinetics of uptake of 99mTc-MIBI and of 99mTc-tetrofosmin was similar between fibrosarcoma and liposarcoma cells, as well as between synovial sarcoma and chondrosarcoma cells. 99mTc-furifosmin showed similar uptake kinetics in all cell lines. The uptake of 99mTc-furifosmin was, however, significantly higher in liposarcoma than in the other cells. The data indicate that the cellular uptake of 99mTc-MIBI, 99mTc-tetrofosmin and 99mTc-furifosmin is dependent on cellular metabolic activity.

摘要

研究并比较了锝-99m 六甲基-2-甲氧基异丁基异腈（MIBI）、99mTc-替曲膦和 99mTc-呋膦在人软组织肉瘤细胞系中的摄取特性。将 99mTc-MIBI、99mTc-替曲膦和 99mTc-呋膦分别在 37℃、32℃和 22℃下与人软组织肉瘤细胞 SW 684（纤维肉瘤）、SW 872（脂肪肉瘤）、SW 982（滑膜肉瘤）和 SW 1353（软骨肉瘤）孵育 10 - 120 分钟后，评估这些示踪剂在人软组织肉瘤细胞中的摄取动力学。99mTc-MIBI、99mTc-替曲膦和 99mTc-呋膦的摄取具有温度依赖性。纤维肉瘤和脂肪肉瘤细胞之间以及滑膜肉瘤和软骨肉瘤细胞之间，99mTc-MIBI 和 99mTc-替曲膦的摄取动力学相似。99mTc-呋膦在所有细胞系中显示出相似的摄取动力学。然而，99mTc-呋膦在脂肪肉瘤中的摄取明显高于其他细胞。数据表明，99mTc-MIBI、99mTc-替曲膦和 99mTc-呋膦的细胞摄取取决于细胞代谢活性。