Higby D J, Mishler J M, Rhomberg W, Nicora R W, Holland J F
Vox Sang. 1975;28(3):243-8. doi: 10.1111/j.1423-0410.1975.tb02763.x.
A total of 33 leukaphereses were performed with the IBM continuous flow centrifuge on 28 normal healthy donors for the purpose of obtaining increased yields of granulocytes for infusion into leukopenic recipients. The pretreatment of donors within a 10- to 12-hour period prior to pheresis with a double dose of dexamethasone and the addition of hydroxyethyl starch to the input line of the continuous flow centrifuge, significantly increased the total quantity and efficiency of granulocyte collected as compared to a donor group receiving a single dose of dexamethasone in addition to hydroxyethyl starch. A mean of 25.5 times 10-9 total granulocytes were collected in addition to an efficiency of 2.11 times 10-9 granulocytes harvested per liter of blood processed in the double-dose-treated donors, in contrast to 19.6 times 10-9 total granulocytes collected and an efficiency of 1.82 times 10-9 granulocytes harvested per liter of blood processed in the single dose donor group. The results of the present study demonstrate that elevated quantities of granulocytes can be collected from normal donors by scheduling a double dose of dexamethasone prior to the pheresis procedure.
为了获得更多粒细胞用于输注给白细胞减少的受者，使用IBM连续流动离心机对28名正常健康供者进行了总共33次白细胞单采术。与仅在连续流动离心机输入管路中添加羟乙基淀粉并接受单剂量地塞米松的供者组相比，在单采术前10至12小时内给供者双倍剂量的地塞米松预处理，显著提高了采集的粒细胞总量和效率。接受双倍剂量治疗的供者平均采集到25.5×10⁹个总粒细胞，每升处理血液收获2.11×10⁹个粒细胞，相比之下，单剂量供者组平均采集到19.6×10⁹个总粒细胞，每升处理血液收获1.82×10⁹个粒细胞。本研究结果表明，通过在单采术程序前安排双倍剂量的地塞米松，可以从正常供者中采集到更多数量的粒细胞。
