Marcaud L, Hayes D
Eur J Biochem. 1979 Jul;98(1):267-73. doi: 10.1111/j.1432-1033.1979.tb13185.x.
Tetrahymena pyriformis which has been starved for 20 h by incubation in buffer, and then deciliated, can regenerate its cilia in about 90 min while still in suspension in non-nutrient medium. The process of reciliation is accompanied by protein synthesis which begins a few minutes after deciliation and by synthesis of ribosomal and messenger RNAs during a period extending from about 1 h to about 3 h after deciliation. Although net synthesis of RNA remains at a very low level until 1 h after deciliation, a qualitative change in the translatable poly(A)-containing messenger RNA content of deciliated cells, and in particular, formation of beta-tubulin mRNA can be detected almost immediately after deciliation.
通过在缓冲液中孵育使其饥饿20小时，然后去纤毛的梨形四膜虫，在仍悬浮于无营养培养基中的情况下，大约90分钟内就能再生其纤毛。纤毛再生过程伴随着蛋白质合成，该过程在去纤毛后几分钟开始，同时在去纤毛后约1小时到约3小时的时间段内会合成核糖体RNA和信使RNA。尽管在去纤毛后1小时之前RNA的净合成一直处于非常低的水平，但去纤毛细胞中可翻译的含聚腺苷酸信使RNA含量会发生质的变化，尤其是β-微管蛋白信使RNA几乎在去纤毛后立即就能被检测到。
