构建一种不会突变为四环素抗性的根癌土壤杆菌C58衍生物。
Construction of a derivative of Agrobacterium tumefaciens C58 that does not mutate to tetracycline resistance.
作者信息
Luo Z Q, Clemente T E, Farrand S K
机构信息
Department of Crop Sciences, University of Illinois at Urbana-Champaign, 61801, USA.
出版信息
Mol Plant Microbe Interact. 2001 Jan;14(1):98-103. doi: 10.1094/MPMI.2001.14.1.98.
Agrobacterium tumefaciens C58 mutates to tetracycline resistance at high frequency, complicating the use of many broad-host-range cloning and binary vectors that code for resistance to this antibiotic as the selection marker. Such mutations are associated with a resistant gene unit, tetC58, that is present in the genome of this strain. By deleting the tetC58 locus, we constructed NTL4, a derivative of C58 that no longer mutates to tetracycline resistance. The deletion had no detectable effect on genetic or physiological traits of NTL4 or on the ability of this strain to transform plants.
根癌土壤杆菌C58高频突变为四环素抗性，这使得许多以对这种抗生素的抗性作为选择标记编码的广宿主范围克隆载体和双元载体的使用变得复杂。此类突变与该菌株基因组中存在的一个抗性基因单元tetC58有关。通过删除tetC58位点，我们构建了NTL4，它是C58的一个衍生物，不再突变为四环素抗性。该缺失对NTL4的遗传或生理特性以及该菌株转化植物的能力没有可检测到的影响。
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