Preparation of Chemocompetent Agrobacterium tumefaciens Cells.

Agrobacterium tumefaciens, a soilborne bacterium known for its natural ability to transfer genetic material into plant cells, revolutionized molecular biology and plant genetic engineering. To harness this transformative potential, researchers require competent Agrobacterium cells capable of efficiently delivering desired genetic constructs into plant hosts. Here, we describe a comprehensive and inexpensive method for obtaining Agrobacterium chemocompetent cells, focusing on key aspects such as cell preparation, transformation efficiency, and optimization.