Parra Samuel, Stange Klein Claudia
Centro de Biología Molecular Vegetal, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
Department of Biology, University of Chile, Santiago, Chile.
Methods Mol Biol. 2025;2911:1-4. doi: 10.1007/978-1-0716-4450-8_1.
Agrobacterium tumefaciens, a soilborne bacterium known for its natural ability to transfer genetic material into plant cells, revolutionized molecular biology and plant genetic engineering. To harness this transformative potential, researchers require competent Agrobacterium cells capable of efficiently delivering desired genetic constructs into plant hosts. Here, we describe a comprehensive and inexpensive method for obtaining Agrobacterium chemocompetent cells, focusing on key aspects such as cell preparation, transformation efficiency, and optimization.
根癌农杆菌是一种土壤细菌，以其将遗传物质自然转移到植物细胞中的能力而闻名，它彻底改变了分子生物学和植物基因工程。为了利用这种变革性潜力，研究人员需要有能力的根癌农杆菌细胞，能够有效地将所需的基因构建体传递到植物宿主中。在这里，我们描述了一种全面且廉价的获得根癌农杆菌化学感受态细胞的方法，重点关注细胞制备、转化效率和优化等关键方面。
