Frigeri C, Schimmer B P
Banting and Best Dept. Medical Research, University of Toronto, ON, Canada.
Endocr Res. 2000 Nov;26(4):1005-9. doi: 10.3109/07435800009048631.
This study explores the basis for the altered function of steroidogenic factor-1 (SF1) in a family of ACTH-resistant Y1 adrenal cell mutants. As determined in electrophoretic mobility shift assays, the DNA binding activity of SF1 was not impaired in the mutant clones. Instead, the ability of SF1 to interact with the coactivator, GRIP1 was affected as determined in a modified mammalian 2-hybrid assay. These findings indicate that the mutants harbor a defect affecting the activation function of SF1.
本研究探讨了促肾上腺皮质激素（ACTH）抵抗性Y1肾上腺细胞突变体系中类固醇生成因子-1（SF1）功能改变的基础。如电泳迁移率变动分析所确定的，SF1的DNA结合活性在突变克隆中未受损。相反，如改良的哺乳动物双杂交分析所确定的，SF1与共激活因子GRIP1相互作用的能力受到了影响。这些发现表明，这些突变体存在影响SF1激活功能的缺陷。
