Fan J G, Wang Q E, Liu S J
National Center for Occupational Safety Science and Technology, Beijing 100029, China.
Biomed Environ Sci. 2000 Sep;13(3):163-9.
It has been verified that asbestos induce neoplastic transformation of several rodent cell lines in vitro. Very little information is available, however, on asbestos-induced human cell line transformation and oncogene activation in the development of asbestos-induced cell transformation. This study was designed to investigate the induction of cell transformation and transcriptional changes of the c-myc oncogene by chrysotile asbestos fibers (CAF) in human embryo lung (HEL) cells. Surface-modified CAF soaked in aluminium citrate (AC), rare earth compounds (REC) or sodium selenite (SS) solutions were used to observe the effects of surface modification on chrysotile-induced cell transformation and transcriptional changes in the c-myc oncogene. The results showed that the natural CAF caused dose-dependent increases in transformed foci, along with increased transcription of the c-myc oncogene in HEL cells, while the pretreated CAF induced fewer transformed foci and less c-myc oncogene transcription than the natural CAF. The percent inhibition of equivalent AC, REC and SS were 38.9%, 50.0%, 33.3% (for transformation focus), 81.6%, 69.5%, 89.9% (for c-myc oncogene transcription), respectively. These results indicated that CAF may induce cell transformation and transcriptional changes and that the carcinogenicity of CAF might be reduced by pretreatment of CAF with the above-mentioned compounds.
已证实石棉可在体外诱导多种啮齿动物细胞系发生肿瘤转化。然而，关于石棉诱导的人细胞系转化以及石棉诱导的细胞转化过程中癌基因激活的信息却非常少。本研究旨在探讨温石棉纤维（CAF）对人胚肺（HEL）细胞的细胞转化诱导作用以及c-myc癌基因的转录变化。使用浸泡在柠檬酸铝（AC）、稀土化合物（REC）或亚硒酸钠（SS）溶液中的表面改性CAF，以观察表面改性对温石棉诱导的细胞转化和c-myc癌基因转录变化的影响。结果表明，天然CAF导致转化灶呈剂量依赖性增加，同时HEL细胞中c-myc癌基因的转录也增加，而预处理后的CAF诱导的转化灶和c-myc癌基因转录均少于天然CAF。等效的AC、REC和SS的抑制率分别为38.9%、50.0%、33.3%（针对转化灶），81.6%、69.5%、89.9%（针对c-myc癌基因转录）。这些结果表明，CAF可能诱导细胞转化和转录变化，并且用上述化合物预处理CAF可能会降低其致癌性。
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