Love D N, Donaldson-Wood C
Arch Virol. 1975;47(2):167-75. doi: 10.1007/BF01320556.
The zwitterionic buffer HEPES was used successfully to maintain organ cultures of kitten tongue and trachea for 10--15 days in vitro. A strain of feline calicivirus grew to high titres in both tongue and tracheal explants but it was not possible to ascribe specific histological changes to virus infection in either tongue or tracheal organ cultures. Cells in tongue explants retained specific virus receptors for at least six days in vitro and virus grew to high levels in the cultures infected six days postinitiation of explants. These results suggest that at least some strains of feline caliciviruses have tropism for lingual epithelium as well as respiratory tract tissues.
两性离子缓冲液HEPES已成功用于在体外维持小猫舌头和气管的器官培养10 - 15天。一种猫杯状病毒株在舌头和气管外植体中均生长至高滴度，但在舌头或气管器官培养物中，无法将特定的组织学变化归因于病毒感染。舌头外植体中的细胞在体外至少保留特定病毒受体六天，并且在接种外植体六天后感染的培养物中病毒生长至高水平。这些结果表明，至少某些猫杯状病毒株对舌上皮以及呼吸道组织具有嗜性。
