Chellegatti M A, Kawano C Y, Said S, Fonseca M J
Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café s/n,-CEP 14040-903-Ribeirão Preto-SP-Brazil.
J Basic Microbiol. 2000;40(5-6):319-26. doi: 10.1002/1521-4028(200012)40:5/6<319::aid-jobm319>3.0.co;2-a.
Penicillium frequentans synthesized eleven polygalacturonases and three pectinesterases when grown in the presence of pectin, sodium polypectate or monogalacturonic acid. When glucose was the sole carbohydrate source in the medium two of these polygalacturonases and one pectinesterase were produced. The enzymes produced under any of these conditions degraded pectic substrates to monogalacturonic acid, suggesting that this monosaccharide or its metabolites should induce the pectinolytic complex. All pectinesterases and most of the extracellular polygalacturonases were synthesized after the 2nd hour of incubation. The pectinases produced by Penicillium frequentans were not secreted at the same time but after 5 hours of incubation all of them could be detected outside the cell those detected only inside the cell were probably membrane-associated or unglycosylated forms of the extracellular pectinases.
在果胶、聚果胶酸钠或半乳糖醛酸存在的条件下生长时，频繁青霉合成了11种聚半乳糖醛酸酶和3种果胶酯酶。当葡萄糖是培养基中唯一的碳水化合物来源时，会产生其中2种聚半乳糖醛酸酶和1种果胶酯酶。在这些条件中的任何一种下产生的酶都会将果胶底物降解为半乳糖醛酸，这表明这种单糖或其代谢产物应该会诱导果胶分解复合物的产生。所有果胶酯酶和大多数细胞外聚半乳糖醛酸酶都是在培养2小时后合成的。频繁青霉产生的果胶酶不是同时分泌的，但在培养5小时后，所有的酶都能在细胞外被检测到，那些仅在细胞内被检测到的可能是细胞外果胶酶的膜相关形式或未糖基化形式。
