携带c-myc标签序列的三磷酸2'-脱氧胞苷的合成与性质
Synthesis and properties of 2'-deoxycytidine triphosphate carrying c-myc tag sequence.
作者信息
Hinz M, Gottschling D, Eritja R, Seliger H
机构信息
Universität Ulm, Sektion Polymere, Germany.
出版信息
Nucleosides Nucleotides Nucleic Acids. 2000 Oct-Dec;19(10-12):1543-52. doi: 10.1080/15257770008045445.
PMID:11200258
Abstract
The synthesis of 2'-deoxycytidine triphosphate carrying mercaptoethyl groups at position 4 of cytosine is described. This nucleoside triphosphate was reacted with a maleimido-peptide carrying the c-myc tag-sequence to yield a peptide-nucleoside triphosphate chimera. Primer extension studies showed that the nucleoside triphosphate modified with the peptide sequence is incorporated by DNA polymerases opposite guanine.
摘要
描述了在胞嘧啶4位带有巯基乙基的2'-脱氧胞苷三磷酸的合成。该核苷三磷酸与携带c-myc标签序列的马来酰亚胺肽反应，生成肽-核苷三磷酸嵌合体。引物延伸研究表明，用肽序列修饰的核苷三磷酸可被DNA聚合酶掺入与鸟嘌呤相对的位置。
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