Matray T, Gamsey S, Pongracz K, Gryaznov S
Geron Corporation, Menlo Park, CA 94025, USA.
Nucleosides Nucleotides Nucleic Acids. 2000 Oct-Dec;19(10-12):1553-67. doi: 10.1080/15257770008045446.
2'-Deoxyribo- and ribo-oligonucleotide N3'-->P5'phosphoramidates containing 2,6-diaminopurine nucleosides were synthesized. Thermal denaturation experiments demonstrated a significant stabilization of the complexes formed by these compounds with DNA and RNA complementary strands, relative to adenosine-containing phosphoramidate counterparts. The increase in melting temperature of the complexes reached up to 6.9 degrees C per substitution. The observed stabilization was attributed to the apparent synergistic effects of N-type sugar puckering of the oligonucleotide N3'-->P5' phosphoramidate backbone, and the ability of 2,6-diaminopurine bases to form three hydrogen bonds.
合成了含有2,6 - 二氨基嘌呤核苷的2'-脱氧核糖和核糖寡核苷酸N3'→P5'氨基磷酸酯。热变性实验表明，相对于含腺苷的氨基磷酸酯类似物，这些化合物与DNA和RNA互补链形成的复合物有显著的稳定性。复合物的解链温度每一次取代增加高达6.9摄氏度。观察到的稳定性归因于寡核苷酸N3'→P5'氨基磷酸酯主链的N型糖折叠以及2,6 - 二氨基嘌呤碱基形成三个氢键的能力所产生的明显协同效应。
