DNA聚合酶催化吡咯甲酰胺核苷三磷酸的合成与掺入。

Synthesis and incorporation of pyrrole carboxamide nucleoside triphosphates by DNA polymerases.

作者信息

Loakes D, Guo M J, Brown D M, Salisbury S A, Smith C L, Felix I, Kumar S, Nampalli S

机构信息

Medical Research Council, Laboratory of Molecular Biology, Cambridge, UK.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2000 Oct-Dec;19(10-12):1599-614. doi: 10.1080/15257770008045449.

DOI:10.1080/15257770008045449

PMID:11200262

Abstract

We have synthesised and examined the enzymatic incorporation properties of the 5'-triphosphates of 2'-deoxyribosyl pyrrole 3-monocarboxamide (dMTP) and 2'-deoxyribosyl pyrrole 3,4-dicarboxamide (dDTP). These analogues we had hoped would behave as ambivalent base analogues in that they can present two alternative hydrogen-bonding faces either by rotation about the carboxamide group or about the glycosidic bond. The two pyrrole derivatives, dMTP and dDTP, exhibit a preference for incorporation with Klenow polymerase. They are preferentially incorporated as either A or C.

摘要

我们已经合成并研究了2'-脱氧核糖基吡咯3-单羧酰胺（dMTP）和2'-脱氧核糖基吡咯3,4-二羧酰胺（dDTP）的5'-三磷酸酯的酶促掺入特性。我们曾希望这些类似物能作为矛盾碱基类似物，因为它们可以通过围绕羧酰胺基团或糖苷键旋转来呈现两个可供选择的氢键结合面。这两种吡咯衍生物，dMTP和dDTP，表现出对klenow聚合酶掺入的偏好。它们优先作为A或C掺入。