Nielsen S D, Sørensen T U, Ersbøll A K, Ngo N, Mathiesen L, Nielsen J O, Hansen J E
Department of Infectious Diseases, Hvidovre Hospital, Denmark.
Scand J Infect Dis. 2000;32(6):597-603. doi: 10.1080/003655400459487.
This study was conducted to determine the impact of immune activation, cytokine production and apoptosis on the naive CD4+ cell count and the function of hematopoietic progenitor cells during the initial phase of highly active antiretroviral therapy (HAART). Blood samples from 11 HIV-infected patients were collected prior to HAART and after 4 and 12 weeks of therapy. Flow cytometry was used to determine the naive CD4+ count and activated T cells. The cloning efficiency of progenitor cells was determined using a colony-forming cells assay. Finally, apoptosis and cytokine production were determined. During the study period, the naive CD4+ count and the cloning efficiency increased significantly. Immune activation was found in HIV-infected patients and decreased during HAART. The level of immune activation correlated negatively with both the naive CD4+ count and the function of progenitor cells. A negative correlation was found between apoptosis and the naive CD4+ count. Alterations in cytokine production during HAART or correlation between cytokine production and the naive CD4+ count or the cloning efficiency of progenitor cells were not detected. In conclusion, immune activation in HIV-infected patients treated with HAART is inversely correlated with the function of progenitor cells and the naive CD4+ count.
本研究旨在确定在高效抗逆转录病毒治疗（HAART）初始阶段，免疫激活、细胞因子产生和细胞凋亡对初始CD4+细胞计数及造血祖细胞功能的影响。收集了11例HIV感染患者在HAART治疗前、治疗4周和12周后的血样。采用流式细胞术测定初始CD4+细胞计数和活化T细胞。使用集落形成细胞试验测定祖细胞的克隆效率。最后，测定细胞凋亡和细胞因子产生情况。在研究期间，初始CD4+细胞计数和克隆效率显著增加。在HIV感染患者中发现了免疫激活，且在HAART治疗期间有所下降。免疫激活水平与初始CD4+细胞计数和祖细胞功能均呈负相关。细胞凋亡与初始CD4+细胞计数之间存在负相关。未检测到HAART治疗期间细胞因子产生的变化，或细胞因子产生与初始CD4+细胞计数或祖细胞克隆效率之间的相关性。总之，接受HAART治疗的HIV感染患者的免疫激活与祖细胞功能和初始CD4+细胞计数呈负相关。
