Dinda A K, Singh C, Dash S C, Tiwari S C, Aggarwal S K, Bhowmik D, Bagga A
Renal Pathology Laboratory, Department of Pathology, All India Institute of Medical Sciences, New Delhi.
J Assoc Physicians India. 2000 Oct;48(10):958-61.
An early accurate etiological categorization of acute renal failure (ARF) into acute glomerulonephritis (AGN), acute tubular necrosis (ATN) and acute interstitial nephritis (AIN) is very important in clinical medicine.
To evaluate the efficacy of a simple, cheap supravital staining method developed in our laboratory, we examined urine of 32 cases of ARF at their initial presentation in oliguric phase, which were later biopsied. The stain consisted of 1% crystal violet and 0.5% safranin in normal saline. The coverslip preparations of coded and stained urine sediments were examined under ordinary bright field microscope (BFM) by two independent observers.
The renal biopsy showed 12 cases of AGN, 12 ATN and 8 AIN. The diagnosis could be predicted by supravital staining method in 75% cases with 95% uniformity among two observers with a sensitivity of 85.7% for AGN followed by AIN (80%) and ATN (75%).
Thus this simple supravital staining technique can be used with ordinary BFM for accurate urine sediment analysis in cases of ARF in bedside medicine.
在临床医学中，将急性肾衰竭（ARF）早期准确病因分类为急性肾小球肾炎（AGN）、急性肾小管坏死（ATN）和急性间质性肾炎（AIN）非常重要。
为评估我们实验室开发的一种简单、廉价的活体染色方法的效果，我们在32例急性肾衰竭患者少尿期初诊时检查了其尿液，这些患者随后接受了活检。该染色剂由生理盐水中1%的结晶紫和0.5%的番红组成。由两名独立观察者在普通明视野显微镜（BFM）下检查编码并染色的尿沉渣盖玻片标本。
肾活检显示12例急性肾小球肾炎、12例急性肾小管坏死和8例急性间质性肾炎。活体染色法在75%的病例中可预测诊断结果，两名观察者之间的一致性为95%，对急性肾小球肾炎的敏感性为85.7%，其次是急性间质性肾炎（80%）和急性肾小管坏死（75%）。
因此，这种简单的活体染色技术可与普通明视野显微镜一起用于床边医学中急性肾衰竭病例的准确尿沉渣分析。
