慢性肾衰竭患者血液透析前后氧化应激的评估

Evaluation of oxidative stress before and after haemodialysis in chronic renal failure.

作者信息

Chugh S N, Jain S, Agrawal N, Sharma A

机构信息

Department of Biochemistry, Pt. BD Sharma PGIMS, Rohtak.

出版信息

J Assoc Physicians India. 2000 Oct;48(10):981-4.

PMID:11200923

Abstract

OBJECTIVE

To evaluate the oxidative stress before and after haemodialysis in chronic renal failure patients.

METHODS

A prospective study comprising of 22 patients of CRF who have to receive their first haemodialysis. All patients were subjected to standard four hours haemodialysis. The parameters of oxidative stress i.e. erythrocyte malonaldehyde (MDA), reduced glutathione (GSH) and superoxide dismutase (SOD) were measured before and after haemodialysis.

RESULTS

The value of mean erythrocyte MDA (9.40 +/- 3.36 mumol/L) and SOD (617 +/- 64.33 units/ml) were significantly higher in patients of CRF before haemodialysis than in controls (p < 0.001). The mean GSH levels were significantly lower (451 +/- 63.91 micrograms/ml) in patients than in controls before haemodialysis (p < 0.001). After haemodialysis MDA levels further increased (12.27 +/- 4.38 mumol/L), SOD levels decreased (458 +/- 69.58 EU/ml) and GSH levels further decreased (396 +/- 41.41 micrograms/ml) (p < 0.001).

CONCLUSIONS

There was an evidence of oxidative stress in patients of CRF before haemodialysis which increased further after haemodialysis, the mechanisms of which is not delineated. The procedural factors may be contributing in the increased oxidative stress after haemodialysis.

摘要

目的

评估慢性肾衰竭患者血液透析前后的氧化应激情况。

方法

一项前瞻性研究，纳入22例首次接受血液透析的慢性肾衰竭患者。所有患者均接受标准的4小时血液透析。分别在血液透析前后检测氧化应激参数，即红细胞丙二醛（MDA）、还原型谷胱甘肽（GSH）和超氧化物歧化酶（SOD）。

结果

慢性肾衰竭患者血液透析前的平均红细胞MDA值（9.40±3.36μmol/L）和SOD值（617±64.33单位/ml）显著高于对照组（p<0.001）。血液透析前患者的平均GSH水平显著低于对照组（451±63.91μg/ml）（p<0.001）。血液透析后，MDA水平进一步升高（12.27±4.38μmol/L），SOD水平降低（458±69.58EU/ml），GSH水平进一步降低（396±41.41μg/ml）（p<0.001）。