Wakamatsu H, Takahashi S, Moriya T, Inouye S T, Okamura H, Akiyama M, Shibata S
Department of Pharmacology and Brain Science, School of Human Sciences, Waseda University, Tokorozawa, Saitama, Japan.
Neuroreport. 2001 Jan 22;12(1):127-31. doi: 10.1097/00001756-200101220-00033.
It is well known that light induces both mPer1 and mPer2 mRNA in the suprachiasmatic nucleus. We have reported that mPer1 antisense oligonucleotides (ODNs) inhibited the light-induced phase delays of mouse locomotor rhythm. In this study, we asked whether both or either mPer1 or mPer2 expression is necessary to induce the phase shift. We examined the effects of inhibition of mRNA expression on light-induced phase delays of mouse circadian behavior rhythm. Light-induced phase delays were moderately attenuated by microinjection of mPer1 or mPer2 antisense ODN, but not by mPer3 antisense or mPer1, mPer2 scrambled ODNs, whereas following simultaneous injection of both mPer1 and mPer2 antisense ODNs they disappeared. The present results suggest that acute induction of mPer1 and mPer2 gene play an additive effect on photic entrainment.
众所周知，光可诱导视交叉上核中的mPer1和mPer2 mRNA。我们曾报道，mPer1反义寡核苷酸（ODNs）可抑制光诱导的小鼠运动节律相位延迟。在本研究中，我们探究了mPer1或mPer2的表达是诱导相位移动所必需的，还是两者均必需。我们研究了mRNA表达抑制对小鼠昼夜行为节律光诱导相位延迟的影响。通过显微注射mPer1或mPer2反义ODN，光诱导的相位延迟会适度减弱，但mPer3反义或mPer1、mPer2随机ODN则不会，而同时注射mPer1和mPer2反义ODN后，光诱导的相位延迟则消失。目前的结果表明，mPer1和mPer2基因的急性诱导对光同步化具有累加效应。
