Taguchi T, Kosaka M, Murakami S, Ohtsuka A, Murakami T
Department of Radiological Technology, Faculty of Health Sciences, Okayama University Medical School, Japan.
Arch Histol Cytol. 2000;63(5):485-94. doi: 10.1679/aohc.63.485.
Electron microscopy of ultrathin sections stained with cationic iron colloid revealed that the rat pineal gland is provided with wide and intensely negative-charged pericapillary spaces. Light microscopically, the negative charging of the pericapillary spaces was completely eliminated by digestion with hyaluronidase and chondroitinase ABC. This pericapillary negative charging was also erased by digestion with collagenase. The results indicate that the negative charging is derived from sulfated proteoglycans which are bound to collagen molecules. These sulfated proteoglycans in the pericapillary spaces may retain numerous water molecules to form a tissue gel, and so act as a selective sieve regulating the passage of tissue molecules.
用阳离子铁胶体染色的超薄切片的电子显微镜检查显示，大鼠松果体具有宽阔且带强负电荷的毛细血管周围间隙。在光学显微镜下，用透明质酸酶和软骨素酶ABC消化可完全消除毛细血管周围间隙的负电荷。用胶原酶消化也可消除这种毛细血管周围的负电荷。结果表明，负电荷源自与胶原分子结合的硫酸化蛋白聚糖。毛细血管周围间隙中的这些硫酸化蛋白聚糖可能保留大量水分子以形成组织凝胶，从而作为调节组织分子通过的选择性筛子。
