来自兔网织红细胞膜结合核糖体的珠蛋白信使核糖核酸
Globin mRNA from rabbit reticulocyte membrane-bound ribosomes.
作者信息
Brennessel B A, Goldstein J
出版信息
Biochim Biophys Acta. 1975 Jan 6;378(1):73-9. doi: 10.1016/0005-2787(75)90138-0.
Messenger RNA has been isolated from both free and membrane-bound rabbit reticulocyte ribosomes. Membrane-bound ribosomes, which constitute less than 10% of total cellular ribosomes, are released from the membrane by deoxycholate but remain associated with the membrane fraction following treatment with 0.5 M KC1. The major messenger RNA species isolated from either free or bound ribosomes is globin messenger. Both alpha- and beta-globin chains are synthesized when the isolated messenger RNA from either free or membrane-bound reticulocyte ribosomes is added to a Krebs ascites cell-free system.
已从游离和膜结合的兔网织红细胞核糖体中分离出信使核糖核酸。膜结合核糖体占细胞总核糖体的比例不到10%，用脱氧胆酸盐可使其从膜上释放，但用0.5M氯化钾处理后仍与膜部分结合。从游离或结合核糖体中分离出的主要信使核糖核酸种类是珠蛋白信使核糖核酸。当将从游离或膜结合的网织红细胞核糖体中分离出的信使核糖核酸添加到克雷布斯腹水无细胞系统中时，α和β珠蛋白链均可合成。
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