Alvarez M G, Marty C, Mori G, Rivarola V
Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta 36-Km 601, (5800) Río Cuarto, Córdoba, Argentina.
Biocell. 2000 Dec;24(3):213-6.
DFMO is an irreversible inhibitor of ornithine decarboxilase (ODC), the key enzyme in mammalian polyamine biosynthesis, and has been shown to induce apoptosis. In this paper, the relation between the effects of DFMO on the polyamine content, apoptotic index and Fas expression in HEP-2 cells was determined. Fas is a type I membrane protein with a molecular mass of 45 kDa, which mediates apoptosis. The results suggest that the treatment with the polyamine inhibitor DFMO induced the expression of the surface antigen Fas, which could be responsible for trigger apoptosis in these cells.
二氟甲基鸟氨酸（DFMO）是鸟氨酸脱羧酶（ODC）的不可逆抑制剂，ODC是哺乳动物多胺生物合成中的关键酶，并且已被证明可诱导细胞凋亡。本文测定了DFMO对HEP-2细胞中多胺含量、凋亡指数和Fas表达的影响之间的关系。Fas是一种分子量为45 kDa的I型膜蛋白，可介导细胞凋亡。结果表明，用多胺抑制剂DFMO处理可诱导表面抗原Fas的表达，这可能是引发这些细胞凋亡的原因。
