Grill V, Sandrucci M A, Di Lenarda R, Basa M, Narducci P, Martelli A M, Bareggi R
Department of Human Morphology, Via Manzoni 16, University of Trieste, I-34138 Trieste, Italy.
Quintessence Int. 2000 Nov-Dec;31(10):741-7.
This short-term (72- to 96-hour) in vitro study on fibroblasts evaluated the biocompatibility of 3 single-phase dental alloys by determining cellular proliferation rates and the expression of a glycoprotein, fibronectin, which is involved in cellular adhesion processes.
Flow 2002 fibroblasts were cultured together with 3 single-phase dental alloys of different composition. Proliferation rates were determined by 5-bromodeoxyuridine incorporation. Fibronectin expression was determined by indirect immunofluorescence.
At 72 hours, cells cultured with the alloy containing the lowest amount of noble elements (gold, platinum, and palladium) and the highest amount of silver exhibited significantly less proliferation than did controls. At 96 hours, only cultures with the alloy containing the greatest amount of noble elements behaved in a way similar to controls. Fibronectin organization in fibrils and in focal adhesions was correlated to higher cellular proliferation rates.
Fibronectin organization could be a useful tool to determine the biocompatibility of dental alloys. Among the noble elements, palladium by itself exhibits very good biocompatibility. These indications could be useful for practitioners in the choice of the best alloy for specific clinical applications.
这项针对成纤维细胞的短期（72至96小时）体外研究，通过测定细胞增殖率以及一种参与细胞黏附过程的糖蛋白——纤连蛋白的表达，评估了3种单相牙科合金的生物相容性。
将Flow 2002成纤维细胞与3种不同成分的单相牙科合金共同培养。通过掺入5-溴脱氧尿苷来测定增殖率。通过间接免疫荧光法测定纤连蛋白的表达。
在72小时时，与含有最低量贵金属元素（金、铂和钯）和最高量银的合金一起培养的细胞，其增殖明显低于对照组。在96小时时，只有与含有最高量贵金属元素的合金一起培养的细胞表现出与对照组相似的行为。纤连蛋白在原纤维和黏着斑中的组织化与较高的细胞增殖率相关。
纤连蛋白的组织化可能是确定牙科合金生物相容性的一种有用工具。在贵金属元素中，钯本身表现出非常好的生物相容性。这些指标可能有助于从业者为特定临床应用选择最佳合金。
