Mommers E C, Poulin N, Meijer C J, Baak J P, van Diest P J
Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
Anal Cell Pathol. 2000;20(4):187-95. doi: 10.1155/2000/965613.
In several tissues, nuclear differences have been described in normal-appearing cells from patients with invasive carcinomas compared to cases without invasive carcinoma, a phenomenon known as malignancy-associated changes (MACs). The aim of this study was to determine the presence of malignancy-associated changes in breast tissue. Image cytometry was performed on Feulgen stained tissue sections of patients with usual ductal hyperplasia with (n = 30) or without (n = 41) adjacent invasive breast carcinoma. Nuclear features of normal-appearing cells as well as of usual ductal hyperplastic cells were separately compared between the two groups. Many features of normal-appearing epithelial cells were significantly different between cases with and without invasive cancer. Significant differences were also found by measuring ductal hyperplastic nuclei instead of normal-appearing nuclei. Cases with or without cancer could be distinguished with a classification accuracy of 80% by discriminant analysis using 2 nuclear features derived from ductal hyperplastic cells. In conclusion, image cytometry on breast tissue sections shows that malignancy-associated changes can be found in normal as well as in usual ductal hyperplastic breast cells. This could be clinically relevant for the detection of occult breast cancer, for the prediction of risk in these lesions, and to monitor the effect of chemopreventive agents.
在多个组织中，与无浸润性癌的病例相比，浸润性癌患者外观正常的细胞中已发现核差异，这一现象被称为恶性肿瘤相关变化（MACs）。本研究的目的是确定乳腺组织中是否存在恶性肿瘤相关变化。对伴有（n = 30）或不伴有（n = 41）相邻浸润性乳腺癌的普通导管增生患者的福尔根染色组织切片进行图像细胞术分析。分别比较了两组中外观正常细胞以及普通导管增生细胞的核特征。有浸润性癌和无浸润性癌的病例中，外观正常的上皮细胞的许多特征存在显著差异。通过测量导管增生细胞核而非外观正常的细胞核，也发现了显著差异。使用源自导管增生细胞的2个核特征进行判别分析，区分有癌和无癌病例的分类准确率可达80%。总之，乳腺组织切片的图像细胞术分析表明，在正常以及普通导管增生的乳腺细胞中均可发现恶性肿瘤相关变化。这在隐匿性乳腺癌的检测、这些病变风险的预测以及监测化学预防剂的效果方面可能具有临床意义。
