Juretić E, Uzarević B, Petrovecki M, Juretić A
Zagreb Clinical Hospital Centre, Department of Obstetrics and Gynecology, University School of Medicine, Croatia.
Immunobiology. 2000 Nov;202(5):421-8. doi: 10.1016/S0171-2985(00)80101-1.
Immune system maturation proceeds postnatally in humans. Therefore, newborns, especially those of a lower gestational age, are not fully immunocompetent and are more likely to acquire perinatal infections. In order to investigate the neonatal immune system status, the major lymphocyte subpopulations were studied in newborns of different gestational age, comparing term newborns and adults. The cord blood from 66 newborns and the peripheral blood from 23 adults were analyzed using fluorochrome labelled monoclonal antibodies and two-color flow cytometry. The newborns were divided into three groups according to their gestational age. Ten prematures were under 32 weeks of gestation, 35 were of 32-37 weeks and there were 21 term newborns. The percentage of cytotoxic T lymphocytes (CD4 CD8+) was lower in term newborns as compared to the adult controls (17.8 versus 30.3%), and so were the percentages of activated T lymphocytes (CD3+Ia+; 0.3 versus 3.7%), cytotoxic non-MHC restricted T lymphocytes (CD3+CD16+CD56+; 0.2 versus 1.8%) and NK cells (CD3-CD16+CD56+; 4.8 versus 15.5%). On the contrary, the proportions of unlabelled cells were increased in term cord blood. The expression of CD45R0 marker on neonatal lymphocytes was very low (1%). In comparison to the higher-gestation newborns, the lower gestation prematures had reduced percentages of T lymphocytes (CD3+; 43 versus 65%), mostly helper T lymphocytes (CD4+CD8-; 35 versus 50%), and increased percentages of unlabelled cells. The percentages of NK cells (CD3+CD16+CD56+) and B lymphocytes (CD3-CD19+; CD3-Ia+) did not differ among the tested newborn groups. There were no significant differences in major lymphocyte subpopulations between the group of highest-gestation prematures and the group of term newborns that differed significantly when compared to adults. The lowest-gestation newborns showed the most immature lymphocyte phenotype with the highest percentages of unlabelled cells.
人类免疫系统在出生后逐渐成熟。因此，新生儿，尤其是孕周较小的新生儿，免疫功能并不完全，更易发生围产期感染。为了研究新生儿免疫系统状态，我们对不同孕周的新生儿主要淋巴细胞亚群进行了研究，并与足月儿和成人进行比较。使用荧光标记单克隆抗体和双色流式细胞术分析了66例新生儿的脐血和23例成人的外周血。根据孕周将新生儿分为三组。10例早产儿孕周小于32周，35例孕周为32 - 37周，21例为足月儿。与成人对照组相比，足月儿中细胞毒性T淋巴细胞（CD4 CD8 +）的百分比更低（17.8%对30.3%），活化T淋巴细胞（CD3 + Ia +；0.3%对3.7%）、细胞毒性非MHC限制性T淋巴细胞（CD3 + CD16 + CD56 +；0.2%对1.8%）和NK细胞（CD3 - CD16 + CD56 +；4.8%对15.5%）的百分比也是如此。相反，足月儿脐血中未标记细胞的比例增加。新生儿淋巴细胞上CD45R0标志物的表达非常低（1%）。与孕周较大的新生儿相比，孕周较小的早产儿T淋巴细胞（CD3 +）百分比降低（43%对65%），主要是辅助性T淋巴细胞（CD4 + CD8 -；35%对50%），未标记细胞的百分比增加。NK细胞（CD3 + CD16 + CD56 +）和B淋巴细胞（CD3 - CD19 +；CD3 - Ia +）的百分比在受试新生儿组之间没有差异。孕周最大的早产儿组与足月儿组之间主要淋巴细胞亚群没有显著差异，与成人相比有显著差异。孕周最小的新生儿表现出最不成熟的淋巴细胞表型，未标记细胞的百分比最高。
