Hon S I, Pyun Y R
Korea Food Research Institute, Kyonggi-do, South Korea.
Int J Food Microbiol. 2001 Jan 22;63(1-2):19-28. doi: 10.1016/s0168-1605(00)00393-7.
Physiological changes of Lactobacillus plantarum (KFRI 815) by high pressure CO2 treatment were investigated to examine the relevance to microbial inactivation. Characteristic properties of the cells measured in this study included salt tolerance, release of UV-absorbing substances, Mg and K ions, proton permeability, glycolysis, H+-ATPase and constitutive enzymes, and dye uptake. The cells treated with high pressure CO2 of 7 MPa at 30 degrees C for 10 min showed the irreversible cellular membrane damages including loss of salt tolerance, leakage of UV-absorbing substances, release of intracellular ions, collapse of proton permeability and uptake of Phloxine B dye. L. plantarum cells after CO2 treatment also exhibited reduced glycolytic activity and inactivation of some constituent enzymes. However, H+-ATPase of the cell membrane maintained its initial specific activity of about 2.50 U/mg protein even though viability of the cells was reduced by several log cycles after high pressure CO2 treatment.
研究了植物乳杆菌（KFRI 815）经高压二氧化碳处理后的生理变化，以检验其与微生物失活的相关性。本研究中测定的细胞特性包括耐盐性、紫外线吸收物质的释放、镁离子和钾离子、质子渗透性、糖酵解、H⁺-ATP酶和组成酶以及染料摄取。在30℃下用7MPa的高压二氧化碳处理10分钟的细胞表现出不可逆的细胞膜损伤，包括耐盐性丧失、紫外线吸收物质泄漏、细胞内离子释放、质子渗透性破坏和焰红B染料摄取。二氧化碳处理后的植物乳杆菌细胞还表现出糖酵解活性降低和一些组成酶失活。然而，即使在高压二氧化碳处理后细胞活力降低了几个对数周期，细胞膜的H⁺-ATP酶仍保持其约2.50 U/mg蛋白质的初始比活性。
