Suo Z, Nesland J M
Department of Pathology, The Norwegian Radium Hospital and Institute for Cancer Research, University of Oslo.
Ultrastruct Pathol. 2000 Nov-Dec;24(6):371-81. doi: 10.1080/019131200750060032.
The expression of EGFR family members was examined by immunohistochemistry in 22 phyllodes tumors, and the results were evaluated together with immunohistochemical findings for proliferation markers Ki67 and BM28, and the tumor suppressor gene product p53. Light and electron microscopy were performed in all cases. Clinical information was obtained from the medial records. We did find that expression of EG FR, c-erbB-3 and c-erbB-4 proteins could be detected in the neoplastic mesenchymal cells, and that the expression increased with increasing malignancy. Increased expressions of Ki67, BM28, p53 and EGFR family members in neoplastic cells were associated with malignancy and unfavorable clinical course. Furthermore, the expression of ER-alpha and PR in the epithelial cells of phyllodes tumors was increased compared to that in normal breast epithelium. Finally, the application of electron microscopy helped to identify a group of malignant tumors, revealing neoplastic cells with characteristic nuclear indentations, as well as an increasing number of myofibroblasts.
通过免疫组织化学检测了22例叶状肿瘤中表皮生长因子受体（EGFR）家族成员的表达，并将结果与增殖标志物Ki67和BM28以及肿瘤抑制基因产物p53的免疫组织化学结果进行了评估。所有病例均进行了光镜和电镜检查。临床信息取自病历。我们确实发现，在肿瘤间充质细胞中可检测到EGFR、c-erbB-3和c-erbB-4蛋白的表达，且表达随恶性程度增加而升高。肿瘤细胞中Ki67、BM28、p53和EGFR家族成员表达增加与恶性程度及不良临床病程相关。此外，与正常乳腺上皮相比，叶状肿瘤上皮细胞中雌激素受体α（ER-α）和孕激素受体（PR）的表达增加。最后，电镜的应用有助于识别一组恶性肿瘤，发现具有特征性核凹陷的肿瘤细胞以及数量增多的肌成纤维细胞。
