Arata Y, Hirabayashi J, Kasai K I
Department of Biological Chemistry, Faculty of Pharmaceutical Sciences, Teikyo University, Kanagawa, Japan.
J Chromatogr A. 2001 Jan 5;905(1-2):337-43. doi: 10.1016/s0021-9673(00)00967-5.
Frontal affinity chromatography is a very useful and simple method to analyze molecular interactions between an analyte and an immobilized ligand by calculating the extent of "retardation" of the elution front. We developed a very simple and efficient data-processing procedure that enables the measurement of very small differences in retardation with precision. This procedure was successfully applied to comparison of the binding properties of recombinant C. elegans galectins for their ligand.
前沿亲和色谱是一种非常有用且简单的方法，通过计算洗脱前沿的“延迟”程度来分析分析物与固定化配体之间的分子相互作用。我们开发了一种非常简单且高效的数据处理程序，能够精确测量延迟的极小差异。该程序已成功应用于比较重组秀丽隐杆线虫半乳糖凝集素与其配体的结合特性。
