da Costa Coutinho H L, Kay H E, Manfio G P, Prata Neves M C, Ribeiro J R, Rumjanek N G, Beringer J E
Embrapa Solos, Rio de Janeiro-RJ, Brazil.
Environ Microbiol. 1999 Oct;1(5):401-8. doi: 10.1046/j.1462-2920.1999.00047.x.
Pyrolysis mass spectrometry (PyMS) and DNA fingerprinting (RAPD and RSalpha hybridization) were used to characterize soybean inoculant strains and root nodule isolates of bradyrhizobia from the Brazilian Cerrado soils. Most isolates were shown to be derived from the inoculant strains on the basis of genotype comparisons by DNA fingerprinting. Phenotypic analysis (using PyMS) of the strains and separately of the polysaccharides derived from them showed that the nodule isolates differed from the parental strains, suggesting adaptation to the Cerrado soil environment. The extent of the differences between the derivatives and inoculant strains was similar for comparisons made on the basis of whole-cell preparations or from the isolated polysaccharides, indicating that the adaptation was caused by changes in the composition of the polysaccharides produced.
热解质谱法（PyMS）和DNA指纹分析（随机扩增多态性DNA和RSα杂交）被用于鉴定巴西塞拉多土壤中大豆接种菌株和缓生根瘤菌的根瘤分离物。通过DNA指纹分析进行基因型比较，结果表明大多数分离物源自接种菌株。对这些菌株以及从它们中分离出的多糖分别进行表型分析（使用PyMS），结果显示根瘤分离物与亲本菌株不同，这表明它们适应了塞拉多土壤环境。基于全细胞制剂或分离出的多糖进行比较时，衍生物与接种菌株之间的差异程度相似，这表明这种适应性是由所产生多糖的组成变化引起的。
