Sztarbała T, Goś R, Góralczyk M, Kedziora J, Błaszczyk J, Sibińska E
Department of Ophthalmology, Military Medical University, ul. Zeromskiego 113, 90-549 Łódź, Poland.
Med Sci Monit. 2000 Mar-Apr;6(2):240-3.
To evaluate clinical, physiochemical and biochemical changes in rabbit vitreous body caused by local injection of sulphur hexafluoride gas.
The volume of fluid vitreous fraction was measured with Na+, K+, Mg2+, Ca2+ levels and full proteins concentration in both vitreous fractions in 24 New Zealand rabbits at 2, 7 and 14 day after SF6 injection. The activity of superoxide dismutase, catalase and malonyl dialdehyde were used to evaluate the activity of antioxidative enzymatic system. Control group consisting of 6 New Zealand rabbits had no experimental procedures.
In the investigated group, the fluid vitreous fraction volume was increased while gelatous one was diminished from 0.08 ml in control group to 0.32 ml in the study group (on day 14). The level of Na+, K+, Mg2+, Ca2+ in the fluid fraction was unchanged. On day 7, we noticed statistically significant increase in protein concentration in comparison with the control group and the study group on 14 day. The activity of superoxide dismutase and catalase as well as the level of malonyl dialdehyde were increased in the fluid vitreous fraction compared to the gelatous one in the control group. After the SF6 injection we did not observe any changes of superoxide dismutase and catalase activities in the gelatous part of vitreous body while in the fluid one there was statistically significant decrease in the enzymatic activity and the MDA level in the whole observation time.
The injected sulphur hexafluoride gas caused the damage of the gelatous vitreous fraction with the increase in the fluid one. The oxygen free radicals might trigger these pathological processes.
评估局部注射六氟化硫气体对兔玻璃体造成的临床、物理化学及生化变化。
在24只新西兰兔注射SF6后的第2、7和14天，测量液体玻璃体部分的体积以及两个玻璃体部分中的Na+、K+、Mg2+、Ca2+水平和总蛋白浓度。使用超氧化物歧化酶、过氧化氢酶和丙二醛的活性来评估抗氧化酶系统的活性。对照组由6只未进行实验操作的新西兰兔组成。
在研究组中，液体玻璃体部分的体积增加，而凝胶状部分减少，从对照组的0.08 ml降至研究组第14天的0.32 ml。液体部分中Na+、K+、Mg2+、Ca2+的水平未发生变化。在第7天，我们注意到与对照组相比，蛋白质浓度有统计学意义的增加，而研究组在第14天蛋白质浓度增加。与对照组凝胶状玻璃体部分相比，液体玻璃体部分中超氧化物歧化酶和过氧化氢酶的活性以及丙二醛的水平均升高。注射SF6后，玻璃体凝胶状部分中超氧化物歧化酶和过氧化氢酶的活性未观察到任何变化，而在液体部分中，在整个观察期内酶活性和丙二醛水平有统计学意义的降低。
注射的六氟化硫气体导致凝胶状玻璃体部分受损，液体部分增加。氧自由基可能引发这些病理过程。
