Zajkowska J M, Hermanowska-Szpakowicz T
Dept. Parasitic Diseases and Neuroinfections, Medical University, ul. Zurawia 14, 15-540 Białystok, Poland.
Med Sci Monit. 2000 Mar-Apr;6(2):278-84.
The evaluation of the changes of lymphocytes: T(CD3), B (CD19), subpopulations CD4, CD8, active lymphocytes CD3 + HLA-DR+, lymphocytes with the receptor for IL2(CD3 + CD25+), NK cells as well as the CD4/CD8 ratio in 30 patients with the early localized (group I n = 7) and early disseminated (group II n = 23) type of Lyme disease, before (examination 1) and after the antibiotic therapy (examination 2) was performed. Group III was composed of 90 healthy people. Measurements were carried out in an COULTER EPIC XL cytoflowmeter, using Becton Dickinson antibodies. Statistical analysis was performed using AnStat software. In the examined groups, a decrease of the subpopulations of CD4, CD8 lymphocytes in comparison with healthy subjects was revealed, as well as a decrease of the CD4/CD8 ratio after treatment. A considerably lower percentage value of active lymphocytes CD3 + HLA-DR+ in both groups and the reduction of the NK subpopulation before and after treatment of early disseminated Lyme disease in comparison with healthy people was observed. The higher percentage values of the lymphocytes with IL-2 receptor were not statistically significant. The indicated essential changes in the subpopulations of T lymphocytes, characterized by a decrease before the antibiotic therapy and by the tendency towards an increase after that therapy of the percentage of CD4, CD8, NK and CD3 + HLA-DR+ lymphocytes in peripheral blood, point out their role in the immunopathogenesis of the Lyme disease. The absence of the complete normalization of the examined parameters after the treatment, on the one hand, may provide evidence for some inertia of the elements of the immune system, on the other hand can also result from too short antibiotic therapy and maintenance of the antigenic stimulation.
对30例早期局限性（I组，n = 7）和早期播散性（II组，n = 23）莱姆病患者在抗生素治疗前（检查1）和治疗后（检查2）淋巴细胞的变化进行评估，包括T（CD3）、B（CD19）淋巴细胞亚群、CD4、CD8、活化淋巴细胞CD3 + HLA - DR +、具有IL2受体的淋巴细胞（CD3 + CD25 +）、NK细胞以及CD4/CD8比值。III组由90名健康人组成。使用Becton Dickinson抗体，在COULTER EPIC XL细胞流量计中进行测量。使用AnStat软件进行统计分析。在所检查的组中，与健康受试者相比，CD4、CD8淋巴细胞亚群减少，治疗后CD4/CD8比值也降低。观察到两组中活化淋巴细胞CD3 + HLA - DR +的百分比值均显著较低，与健康人相比，早期播散性莱姆病治疗前后NK亚群减少。具有IL - 2受体的淋巴细胞较高百分比值无统计学意义。外周血中T淋巴细胞亚群的上述显著变化，其特征为抗生素治疗前百分比降低，治疗后CD4、CD8、NK和CD3 + HLA - DR +淋巴细胞百分比有增加趋势，表明它们在莱姆病免疫发病机制中的作用。治疗后检查参数未完全恢复正常，一方面可能证明免疫系统某些成分存在一定惰性，另一方面也可能是由于抗生素治疗时间过短以及抗原刺激持续存在所致。
