Kedzierska A, Turowski G
Independent Laboratory of Clinical Immunology, Faculty of Medicine, Collegium Medicum, Jagiellonian University, ul. Grzegórzecka 16, 31-531 Cracow, Poland.
Med Sci Monit. 2000 Sep-Oct;6(5):957-63.
The aim of the study was the analysis of the frequency of HLA class I antigens in the Polish population of children suffering from coeliac disease and their families, as well as the estimation of the relative risk of incidence, etiologic fraction (EF) and preventive fraction (PF) indexes. Forty-six probands' families were included in the typing: 69 children with coeliac disease confirmed clinically and histologically, 49 healthy siblings and 91 parents. The HLA antigens were typed with routine Terasaki an McClelland's two-stage microcytotoxic assay in NIH modification. The following antigens occurred significantly more frequently (p < 0.0000...1) in phenotypes of children with coeliac disease: HLA-A1 (chi 2 = 35.90; RR = 4.3; EF = 0.44), -B8 (chi 2 = 88.20; RR = 8.8; EF = 0.58) and Cw7 (chi 2 = 55.24; RR = 7.5; EF = 0.69). The positive correlation for the specificity of HLA-A1, -B8 was proved also in siblings (chi 2 = 16.03; chi 2 = 18.10) and parents (chi 2 = 15.67; chi 2 = 32.67). The presence of antigens HLA-A1, -B8 in the phenotype may be the risk factor predisposing for the manifestation of hypersensitivity to gluten.
本研究的目的是分析患有乳糜泻的波兰儿童及其家庭成员中I类HLA抗原的频率，以及估计发病的相对风险、病因分数（EF）和预防分数（PF）指数。46个先证者家庭纳入分型：69名经临床和组织学确诊的乳糜泻儿童、49名健康同胞和91名父母。采用美国国立卫生研究院改良的常规寺崎和麦克莱兰两阶段微量细胞毒性试验对HLA抗原进行分型。在乳糜泻儿童的表型中，以下抗原出现的频率显著更高（p < 0.0000...1）：HLA - A1（χ2 = 35.90；RR = 4.3；EF = 0.44）、- B8（χ2 = 88.20；RR = 8.8；EF = 0.58）和Cw7（χ2 = 55.24；RR = 7.5；EF = 0.69）。在同胞（χ2 = 16.03；χ2 = 18.10）和父母（χ2 = 15.67；χ2 = 32.67）中也证实了HLA - A1、- B8特异性的正相关性。表型中存在抗原HLA - A1、- B8可能是易患麸质超敏反应表现的危险因素。
