Muñoz A, DeFelipe J, Jones E G
Center for Neuroscience, University of California, Davis, CA 95616, USA.
Cereb Cortex. 2001 Feb;11(2):104-13. doi: 10.1093/cercor/11.2.104.
In situ hybridization histochemistry and immunocytochemistry were used to examine GABA(B)R1a,b receptor mRNA and protein expression in areas 17 and 18 of the visual cortex of normal macaque monkeys and of monkeys that had been deprived of vision in one eye. In addition, the normal patterns of GABA(B)R1a,b protein expression were immunocytochemically studied in the human visual cortex. Overall levels of GABA(B)R1a,b transcript were higher in area 17 than in area 18. In area 17 GABA(B)R1a,b mRNA levels were highest in layers IVC and VI, moderate in layers II-IVA and low in layers I, IVB and V. In area 18 GABA(B)R1a,b transcript expression was high in layers II and III, moderate in layers IV and VI and low in layers I and V. Immunocytochemistry revealed nearly identical patterns of GABA(B)R1a,b protein expression in areas 17 and 18 in monkey and human. Both pyramidal and non-pyramidal neurons were GABA(B)R1a,b immunoreactive. The majority of intensely immunoreactive neurons in layers II, III, V and VI were pyramidal cells. Numerous non-pyramidal cells were intensely immunoreactive in layer IV of area 17 but layer IV cells were only lightly immunoreactive in area 18. Following 10 day periods of monocular deprivation, induced by intravitreal injections of tetrodotoxin, levels of GABA(B)R1a,b mRNA and protein were decreased in the deprived eye dominance columns of layers IVC and VI.
采用原位杂交组织化学和免疫细胞化学方法，检测正常猕猴和单眼剥夺猕猴视皮层17区和18区GABA(B)R1a,b受体mRNA和蛋白表达。此外，还采用免疫细胞化学方法研究了人类视皮层GABA(B)R1a,b蛋白表达的正常模式。GABA(B)R1a,b转录本的总体水平在17区高于18区。在17区，GABA(B)R1a,b mRNA水平在IVC层和VI层最高，在II-IVA层中等，在I层、IVB层和V层较低。在18区，GABA(B)R1a,b转录本表达在II层和III层较高，在IV层和VI层中等，在I层和V层较低。免疫细胞化学显示，猕猴和人类17区和18区GABA(B)R1a,b蛋白表达模式几乎相同。锥体细胞和非锥体细胞均为GABA(B)R1a,b免疫反应阳性。II层、III层、V层和VI层中大多数强免疫反应阳性神经元为锥体细胞。17区IV层有大量非锥体细胞呈强免疫反应阳性，但18区IV层细胞仅呈轻度免疫反应阳性。在玻璃体内注射河豚毒素诱导单眼剥夺10天后，IVC层和VI层被剥夺眼优势柱内GABA(B)R1a,b mRNA和蛋白水平降低。
