酶温度活性谱的实验进化：嗜热栖热菌鸟氨酸氨甲酰基转移酶的体内选择及低温适应突变体的表征
Experimental evolution of enzyme temperature activity profile: selection in vivo and characterization of low-temperature-adapted mutants of Pyrococcus furiosus ornithine carbamoyltransferase.
作者信息
Roovers M, Sanchez R, Legrain C, Glansdorff N
机构信息
Department of Microbiology, Flanders Interuniversity Institute for Biotechnology (VIB), B-1070 Brussels, Belgium.
出版信息
J Bacteriol. 2001 Feb;183(3):1101-5. doi: 10.1128/JB.183.3.1101-1105.2001.
Abstract
We have obtained mutants of Pyrococcus furiosus ornithine carbamoyltransferase active at low temperatures by selecting for complementation of an appropriate yeast mutant after in vivo mutagenesis. The mutants were double ones, still complementing at 15 degrees C, a temperature already in the psychrophilic range. Their kinetic analysis is reported.
摘要
我们通过在体内诱变后选择合适的酵母突变体进行互补，获得了在低温下具有活性的嗜热栖热菌鸟氨酸氨甲酰基转移酶突变体。这些突变体是双重突变体，在15摄氏度时仍具有互补作用，该温度已处于嗜冷菌范围内。本文报道了它们的动力学分析。
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