Hayase K, Tanaka M, Tujioka K, Hirano E, Habuchi O, Yokogoshi H
Department of Home Economics, Aichi University of Education, Kariya, Aichi 448-8542, Japan.
J Nutr. 2001 Jan;131(1):123-6. doi: 10.1093/jn/131.1.123.
The purpose of this study was to determine whether 17-ss-estradiol affects the rate of brain protein synthesis in ovariectomized female rats. Experiments were conducted on three groups of 12-wk-old female rats: group 1 were ovariectomized to reduce the level of plasma estradiol, group 2 were ovariectomized and treated with estradiol and group 3 were sham-operated controls. The fractional rates of protein synthesis in brain of ovariectomized rats treated with estradiol were significantly greater than that in ovariectomized rats without estradiol treatment. In the cerebral cortex and cerebellum, the RNA activity [g protein synthesized/(g RNA. d)] significantly correlated (r > 0.87, P < 0.001) with the fractional rate of protein synthesis. The RNA concentration (mg RNA/g protein) was not related to the fractional rate of protein synthesis in any organ. The results suggest that estrogen treatment of ovariectomized female rats is likely to increase the rate of protein synthesis in the brain and that RNA activity is at least in part related to the fractional rate of brain protein synthesis.
本研究的目的是确定17-β-雌二醇是否会影响去卵巢雌性大鼠的脑蛋白合成速率。对三组12周龄雌性大鼠进行了实验：第1组大鼠接受卵巢切除术以降低血浆雌二醇水平，第2组大鼠接受卵巢切除术并给予雌二醇治疗，第3组为假手术对照组。接受雌二醇治疗的去卵巢大鼠脑内的蛋白合成分数率显著高于未接受雌二醇治疗的去卵巢大鼠。在大脑皮层和小脑中，RNA活性[合成的蛋白克数/（克RNA·天）]与蛋白合成分数率显著相关（r>0.87，P<0.001）。RNA浓度（毫克RNA/克蛋白）与任何器官中的蛋白合成分数率均无关。结果表明，对去卵巢雌性大鼠进行雌激素治疗可能会提高脑内蛋白合成速率，并且RNA活性至少部分与脑蛋白合成分数率相关。
