用二甲基亚砜对天然和重组烟草花叶病毒进行脱壳：脱壳的极性
The uncoating of native and reconstituted TMV by dimethylsulphoxide: the polarity of stripping.
作者信息
Nicolaieff A, Lebeurier G, Morel M C, Hirth L
出版信息
J Gen Virol. 1975 Mar;26(3):295-306. doi: 10.1099/0022-1317-26-3-295.
The effect of increasing concentrations of dimethylsulphoxide (DMSO) on the stability of native and reconstituted tobacco mosaic virus (TMV) has been investigated by means of electron microscopy and infectivity assay. The removal of protein subunits from TMV by DMSO was found to be a stepwise process. Subunits are first removed from the 3'OH end of the rod but at about 72% DMSO the site at which uncoating occurs shifts to the 5' end. Studies with reconstituted and partially reconstituted TMV confirm the stepwise nature of the uncoating process. Complete uncoating of reconstituted TMV was found to occur at smaller DMSO concentrations than that of native TMV.
通过电子显微镜和感染性测定，研究了二甲基亚砜（DMSO）浓度增加对天然和重组烟草花叶病毒（TMV）稳定性的影响。发现DMSO从TMV中去除蛋白质亚基是一个逐步的过程。亚基首先从杆状病毒的3'OH末端去除，但在约72%的DMSO时，脱壳发生的位点转移到5'末端。对重组和部分重组TMV的研究证实了脱壳过程的逐步性质。发现重组TMV在比天然TMV更低的DMSO浓度下完全脱壳。
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