Bizet K, Gabrielli C, Perrot H
UPR15 du CNRS, Physique des Liquides et Electrochimie, Université Pierre et Marie Curie, Tour 22, 4, Place Jussieu, 75252 Paris, France.
Appl Biochem Biotechnol. 2000 Nov-Dec;89(2-3):139-49. doi: 10.1385/abab:89:2-3:139.
Biodetection is one of the most important challenges for the twenty-first century: many fields are concerned, mainly environmental and medical. The quartz crystal microbalance (QCM) may offer great possibilities for this purpose: a direct response signal, which characterizes the binding event between a sensitive layer, immobilized onto the surface transducer, and the analyte to be detected, can be obtained. However, for the detection of small biomolecules such as antigens, it is quite difficult to obtain an observable signal that corresponds directly to the binding event. In general, this is owing to the lack of mass sensitivity of the commonly used QCM, with 5- to 10-MHz quartz crystals. For improving this mass sensitivity, a 27-MHz quartz resonator was developed and incorporated in a flow-through microcell. Two biospecies, IgG rabbit and peroxidase enzyme, were studied with this ultra-sensitive QCM in terms of specificity, detection limit, and calibration curve.
生物检测是21世纪最重要的挑战之一：涉及许多领域，主要是环境和医学领域。石英晶体微天平（QCM）可能为此提供极大的可能性：可以获得一个直接响应信号，该信号表征固定在表面换能器上的敏感层与待检测分析物之间的结合事件。然而，对于检测诸如抗原之类的小生物分子，很难获得直接对应于结合事件的可观察信号。一般来说，这是由于常用的5至10MHz石英晶体QCM缺乏质量灵敏度。为了提高这种质量灵敏度，开发了一种27MHz的石英谐振器，并将其集成到流通式微池中。使用这种超灵敏QCM研究了两种生物物种，即兔IgG和过氧化物酶，研究内容包括特异性、检测限和校准曲线。
