Zhong H, Cao C, Li P, Zhang Y H, Shi Y L, Ma Q J
Molecular Genetics Key Laboratory, Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100850, China.
Yi Chuan Xue Bao. 2000;27(11):966-71.
The immunogenicity and protective efficacy of the DNA vaccine which include cholera toxin B subunit (CTB) and polyvalent protective epitopes of Plasmodium falciparum (awte gene) was assessed using rhesus monkeys as animal models. Recombinant plasmids of pCMV-CTB-AWTE were given to five rhesus monkeys three times with two weeks intervals by intramuscle (i.m.) route, immunization dose was 500 micrograms per plasmid per animal. High levels of anti-CTB and anti-malaria epitopes antibodies and P. falciparum epitope specific CTL activity were elicited. The vaccinated groups was challenged with 1.25 x 10(8) of P. cynomolgi parasites. All monkeys of the control group was patent for at least 34 days, the DNA vaccinated groups wasn't infected during the 60 days we detected. The cocktail DNA vaccine which contains multi-stage and multi-epitope antigen gene shows excellent immunogenicity and protective efficacy, the results also suggests that DNA vaccine plays an important role against malaria infection.
以恒河猴为动物模型，评估了包含霍乱毒素B亚基（CTB）和恶性疟原虫多价保护性表位（awte基因）的DNA疫苗的免疫原性和保护效果。将重组质粒pCMV-CTB-AWTE通过肌肉注射（i.m.）途径，每隔两周给5只恒河猴接种3次，每只动物每次接种剂量为500微克质粒。诱导产生了高水平的抗CTB和抗疟疾表位抗体以及恶性疟原虫表位特异性CTL活性。对接种组用1.25×10⁸ 食蟹猴疟原虫进行攻击。对照组所有猴子至少34天呈阳性，在我们检测的60天内，DNA接种组未被感染。包含多阶段和多表位抗原基因的混合DNA疫苗显示出优异的免疫原性和保护效果，结果还表明DNA疫苗在抗疟疾感染中发挥重要作用。
