Montanaro L, Cavedagna D, Baldassarri L, Arciola C R
Research Laboratory on Biocompatibility of Implant Materials, Rizzoli Orthopedic Institute, Bologna, Italy.
New Microbiol. 2001 Jan;24(1):57-61.
Bacterial adhesion to polymethylmethacrylate and to silicon elastomer, materials frequently used in clinical applications, has been investigated to assess whether adhesion selects methicillin-resistant mutants in the bacterial population in contact with the materials. The methicillin susceptibility of a susceptible Staphylococcus aureus (ATCC 25923) was measured by a modification of plate antibiogram Kirby-Bauer method, which allows optimised detection of small variations in antibiotic susceptibility. In both adherent and non-adherent bacterial subpopulations, the presence of mecA gene, which encodes for the protein PBP 2a responsible for methicillin resistance was searched for by Polymerase Chain Reaction (PCR). The contact with the two polymers did not induce in the bacteria population any phenotypic increase in methicillin resistance, or the selection of mutants carrying the mecA gene.
已经对细菌在聚甲基丙烯酸甲酯和硅橡胶（临床应用中常用的材料）上的粘附进行了研究，以评估粘附是否会在与这些材料接触的细菌群体中选择耐甲氧西林突变体。通过改良的平板抗菌谱 Kirby-Bauer 方法测量了敏感金黄色葡萄球菌（ATCC 25923）对甲氧西林的敏感性，该方法能够优化检测抗生素敏感性的微小变化。在粘附和未粘附的细菌亚群中，通过聚合酶链反应（PCR）寻找编码负责耐甲氧西林的蛋白质 PBP 2a 的 mecA 基因的存在。与这两种聚合物的接触并未在细菌群体中诱导出任何甲氧西林耐药性的表型增加，也未选择携带 mecA 基因的突变体。
