Kim J W, Min Y G, Rhee C S, Lee C H, Koh Y Y, Rhyoo C, Kwon T Y, Park S W
Department of Otolaryngology--Head and Neck Surgery, Seoul National University College of Medicine, Korea.
Laryngoscope. 2001 Feb;111(2):246-50. doi: 10.1097/00005537-200102000-00011.
This study was performed to investigate the changes in ciliary beat frequency (CBF) after treatment with Larginine in the human sinus mucosa and to determine the distribution of inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) in the healthy sinus mucosa.
STUDY DESIGN/METHODS: CBF was measured in the sphenoid sinus mucosa of 12 patients who underwent trans-septal trans-sphenoidal hypophysectomy for the treatment of pituitary gland tumor. CBF was measured over 24 hours in Dulbecco's modified Eagle's medium (DMEM) after treatment with L-arginine, its inactive spatial isomer D-arginine, or an NOS inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME). DMEM without treatment with these materials was used as a control. Other pieces of the mucosa were exposed to L-NAME and its inactive spatial isomer D-NAME after preincubation with L-arginine. The specimens were immunohistochemically stained for iNOS and eNOS.
CBF increased 24 hours after treatment with L-arginine as compared with control groups. CBF increased in proportion to the increasing concentrations of L-arginine. There was no significant change after treatment with D-arginine or L-NAME. CBF increased after treatment with L-arginine at 30 minutes and maintained for 24 hours. L-NAME inhibited the increase in CBF by L-arginine, but D-NAME showed no such effect. Immunoreactivity to both iNOS and eNOS was frequently observed in the ciliated epithelial cells and was stronger to eNOS than to iNOS.
From the results of this study it is suggested that nitric oxide (NO) produced by iNOS and eNOS using L-arginine may increase CBF in the healthy sinus mucosa and that NO may have a regulatory function in ciliary motility in the human sinus mucosa.
本研究旨在探讨精氨酸治疗后人鼻窦黏膜中纤毛摆动频率（CBF）的变化，并确定诱导型一氧化氮合酶（iNOS）和内皮型一氧化氮合酶（eNOS）在健康鼻窦黏膜中的分布。
研究设计/方法：对12例因垂体瘤接受经鼻中隔经蝶窦垂体切除术的患者的蝶窦黏膜进行CBF测量。在用L-精氨酸、其无活性的空间异构体D-精氨酸或一氧化氮合酶抑制剂N（G）-硝基-L-精氨酸甲酯（L-NAME）处理后，于杜氏改良 Eagle 培养基（DMEM）中在24小时内测量CBF。未用这些物质处理的DMEM用作对照。在用L-精氨酸预孵育后，将其他黏膜样本暴露于L-NAME及其无活性的空间异构体D-NAME。对标本进行iNOS和eNOS的免疫组织化学染色。
与对照组相比，L-精氨酸处理后24小时CBF增加。CBF随L-精氨酸浓度增加而呈比例增加。用D-精氨酸或L-NAME处理后无显著变化。L-精氨酸处理30分钟后CBF增加并持续24小时。L-NAME抑制L-精氨酸引起的CBF增加，但D-NAME无此作用。在纤毛上皮细胞中经常观察到对iNOS和eNOS的免疫反应性，且对eNOS的反应性强于对iNOS的反应性。
从本研究结果提示，iNOS和eNOS利用L-精氨酸产生的一氧化氮（NO）可能增加健康鼻窦黏膜中的CBF，且NO可能在人鼻窦黏膜的纤毛运动中具有调节功能。
