Liu J R, McKenzie C A, Seviour E M, Webb R I, Blackall L L, Saint C P, Seviour R J
Int J Syst Evol Microbiol. 2001 Jan;51(Pt 1):195-202. doi: 10.1099/00207713-51-1-195.
Five strains of the filamentous bacterium 'Nostocoida limicola' III were successfully isolated into pure culture from samples of activated sludge biomass from five plants in Australia. 16S rRNA gene sequence analyses showed that all isolates were members of the Planctomycetales, most closely related to Isosphaera pallida, but they differed phenotypically from this species in that they did not glide and were not thermotolerant. The ultrastructure of these 'N. limicola' III isolates was also consistent with them being Planctomycetales, in that they possessed complex intracellular membrane systems compartmentalizing the cells. However, the arrangements of these intracellular membranes differed between isolates. These data confirm that 'N. limicola' III is phylogenetically unrelated to both 'N. limicola' I and 'N. limicola' II, activated sludge filamentous bacteria which share morphological features in common with 'N. limicola' III and which have been presumed historically to be the same or very similar bacteria.
从澳大利亚五家工厂的活性污泥生物质样本中，成功分离出了五株丝状细菌“嗜盐碱红杆菌”III，并将其培养成纯培养物。16S rRNA基因序列分析表明，所有分离株均属于浮霉菌目，与苍白等球藻关系最为密切，但在表型上与该物种不同，即它们不会滑行且不耐热。这些“嗜盐碱红杆菌”III分离株的超微结构也与它们属于浮霉菌目一致，因为它们拥有将细胞分隔开的复杂细胞内膜系统。然而，这些细胞内膜的排列在不同分离株之间有所不同。这些数据证实，“嗜盐碱红杆菌”III在系统发育上与“嗜盐碱红杆菌”I和“嗜盐碱红杆菌”II均无关联，后两者是活性污泥丝状细菌，与“嗜盐碱红杆菌”III具有共同的形态特征，并且在历史上一直被认为是相同或非常相似的细菌。
