恒河猴和食蟹猴的淋巴细胞培养物。
Lymphocyte cultures of Macaca mulatta and Macaca fascicularis.
作者信息
De Vries G F, Geleijnse M E, De France H F, Hogendoorn A M
出版信息
Lab Anim Sci. 1975 Feb;25(1):33-8.
PMID:1121162
Abstract
Lymphocytes of Macaca mulatta and Macaca fascicularis were cultured using a microculture technic. The use of RPMI-1640 with fetal calf serum as a culture medium and an extra dose of phytohemagglutinin produced excellent results. Staining of the chromosomes by the Gemsa banding technic did not reveal any differences between the karyotypes of the Macaca species.
摘要
使用微量培养技术培养恒河猴和食蟹猴的淋巴细胞。以含胎牛血清的RPMI - 1640作为培养基，并额外添加植物血凝素，取得了极佳的效果。采用吉姆萨显带技术对染色体进行染色，未发现这两种猕猴的核型之间存在任何差异。
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