Scholz S, Lörz H, Lütticke S
Center for Applied Plant Molecular Biology (AMP II), University of Hamburg, Germany.
Mol Gen Genet. 2001 Jan;264(5):653-61. doi: 10.1007/s004380000351.
Transposition of the maize autonomous element Ac (Activator) was investigated in barley (Hordeum vulgare L.) with the aim of developing a transposon tagging system for the latter. The Ac element was introduced into meristematic tissue of barley by microprojectile bombardment. Transposon activity was then examined in the resulting transgenic plants. Multiple excision events were detected in leaf tissue of all plant lines. The mobile elements generated empty donor sites with small DNA sequence alterations, similar to those found in maize. Reintegration of Ac at independent genomic loci in somatic tissue was demonstrated by isolation of new element-flanking regions by AIMS-PCR (amplification of insertion-mutagenized sites). In addition, transmission of transposed Ac elements to progeny plants was confirmed. The results indicate that the introduced Ac element is able to transpose in barley. This is a first step towards the establishment of a transposon tagging system in this economically important crop.
为了开发一种用于大麦的转座子标签系统，对玉米自主元件Ac（激活子）在大麦（Hordeum vulgare L.）中的转座进行了研究。通过微粒轰击将Ac元件导入大麦的分生组织。然后在所得的转基因植物中检测转座子活性。在所有株系的叶片组织中均检测到多次切除事件。这些移动元件产生了带有小DNA序列改变的空供体位点，类似于在玉米中发现的情况。通过AIMS-PCR（插入诱变位点的扩增）分离新的元件侧翼区域，证明了Ac在体细胞组织中独立基因组位点的重新整合。此外，还证实了转座的Ac元件向后代植株的传递。结果表明，导入的Ac元件能够在大麦中转座。这是在这种经济上重要的作物中建立转座子标签系统的第一步。
